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No stimulation was seen with either untreated or neuraminidase-treated neuraminidase-treated PBMC gangliosides, indicating that the positive response was due to gangliosides with a terminal N-acetyl neuraminic acid. The stimulation was due mainly to the two following sialylated gangliosides: GM3 (Gal beta 1-4[NeuAca2-3]Gal alpha 1-4Glc beta 1-1Cer) and GD1a (NeuAca2-3Gal alpha 1-4Gal beta 1-4Glc beta 1-1Cer). These two gangliosides are present in the range of 100-200 ng/10(6) PBMC, or about 1/3 of total cellular gangliosides. 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